Halal Research Center of Islamic Republic of Iran. Tel.: +98-21-88909033 Fax: +98-21-88890858 Email: jhhhm@halal.ac.ir IR.MCIG No. 85915 Human, Health and Halal Metrics 2717-1027 5 2 2024 12 01 Phytochemical screening of ethanol extract of Ziziphus spina-christi (leaf) and Euphorbia hirta (whole plant) and their analgesic activity in Wistar albino mice 25 32 210877 10.30502/jhhhm.2024.476618.1096 EN Audu Nuhu Tarfa Department of Public Health, Sechenov University, Moscow, First Moscow State Medical University, Moscow, Russian Federation. Hafsat Ali Saab Department of Pharmacology and Toxicology, University of Maiduguri Bama Road, Borno State, Nigeria. Fave Yohanna Tata Department of Pharmacology and Toxicology, University of Maiduguri Bama Road, Borno State, Nigeria. Ayuba Nuhu Tarfa Geneva Center of Humanitarian Studies, University of Geneva, Geneva, Switzerland. Lilian Chukwu Department of Public Health, Sechenov University, Moscow, First Moscow State Medical University, Moscow, Russian Federation. Dorcas Oluwakemi Aremu Department of Public Health, Sechenov University, Moscow, First Moscow State Medical University, Moscow, Russian Federation. 0000-0002-6515-5551 Journal Article 2024 09 03 Background and Objective: Pain is a significant global healthcare problem, usually resulting from either medical procedure, injury, or illness. It can be chronic or acute. In this study, the analgesic effect of the ethanol extract of Euphorbia hirta and Ziziphus spina-christi at different concentrations in Wistar albino mice was determined. In addition, the phytochemical composition of the extracts was studied in the laboratory. Materials and Methods: Ethanol extracts of Euphorbia hirta and Ziziphus spina-christi at concentrations of 250, 500, and 1000 mg/kg were administered to mice, and the results were compared with negative (10 ml/kg distilled water) and positive (30 mg/kg paracetamol) controls using hot plate method. The experiments were done by 24 mice for lethal dose (LD50) study, and 24 mice for the main study. The administration was done via oral route, and the mice were placed on hot plate after every 30 min of administration (six mice in each group). Result and Conclusion: The plants contained tannins, flavonoids, carbohydrates, anthraquinone, saponins, and alkaloids, but triterpenoids were not detected. No acute toxicity or mortality was found in each group after 24 h administration of 10, 100, 1000, 1600, 2900, and 5000 mg/kg body weight of the extracts. The plants had lethal dose (LD50) of ≥ 5000 mg/kg, and Euphorbia hirta had more significant analgesic effects than Ziziphus spina-christi. After administration, the mice were be placed immediately on a hot plate to observe how many minutes they will jump off the hot plate due to the heat. The highest extended mean time reaction for Euphorbia hirta was at the 30th min at a dose of 1000 mg/kg (3.97 ±0.27 min), while that of Ziziphus spina-christi was at the 90th min at a dose of 1000 mg/kg (2.22 ±0.41 min). We conclude that both extracts are potent candidate as natural analgesic agent in pain alleviation. However, more toxicological studies are required.

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